Plasmid

Part:BBa_K4761428:Design

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-30)


pTarget-pykF4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2065
    Illegal XbaI site found at 2071
    Illegal SpeI site found at 1955
    Illegal PstI site found at 1973
    Illegal PstI site found at 2083
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2065
    Illegal NheI site found at 1932
    Illegal SpeI site found at 1955
    Illegal PstI site found at 1973
    Illegal PstI site found at 2083
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2065
    Illegal BglII site found at 2095
    Illegal BamHI site found at 1920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2065
    Illegal XbaI site found at 2071
    Illegal SpeI site found at 1955
    Illegal PstI site found at 1973
    Illegal PstI site found at 2083
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2065
    Illegal XbaI site found at 2071
    Illegal SpeI site found at 1955
    Illegal PstI site found at 1973
    Illegal PstI site found at 2083
    Illegal NgoMIV site found at 771
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References